Translational_Unit
Part:BBa_K1500008:Design
Designed by: Kevin Yang Group: iGEM14_UChicago (2014-10-17)
IPTG-GFP mutated MutY
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 870
Design Notes
NA
Source
It was cloned from wild-type DH5-alpha E. coli sequence and site-directed mutagenesis was performed in the IPTG-GFP plasmid.