Translational_Unit

Part:BBa_K1500008:Design

Designed by: Kevin Yang   Group: iGEM14_UChicago   (2014-10-17)


IPTG-GFP mutated MutY


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 870


Design Notes

NA


Source

It was cloned from wild-type DH5-alpha E. coli sequence and site-directed mutagenesis was performed in the IPTG-GFP plasmid.

References